Coding

Part:BBa_M1699:Design

Designed by: S. Ivan Bartlett   Group: UtahState_BE5930_-_S11   (2013-04-15)


scrT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 490
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 797


Design Notes

This sequence was put into DNA 2.0's Gene Designer software to optimize its G-C content for use in Lactobacillus Johnsonii. Also it was checked for comparability with standard 10 restriction enzymes. The sequence here does not contain any restriction site conflicts with standard 10.


Source

http://www.ncbi.nlm.nih.gov/protein/22550282

References